Transcriptome and Re-Sequencing Analyses Reveal Photosynthesis-Related Genes Involvement in Lutein Accumulation in Yellow Taproot Mutants of Carrot

Carrots accumulate numerous carotenoids in the root, resulting different colors. Orange carrots are primarily high α- and β-carotene, while yellow packed with lutein. This study was designed to explore molecular mechanism underlying mutation involving lutein using a recently obtained root mutant carrot (ym) via mutagenesis of an orange wild type (wt). Microscopes were used observe variations histological cellular structures, transcriptome resequencing analyses conducted for ym wt. The callus contained fewer colored crystals globular chromoplasts than those Based on ribonucleic acid sequencing (RNA-seq) data analysis, 19 photosynthesis-related differentially expressed genes (DEGs) enriched. Among them, there 6 experiencing nonsynonymous mutations, including PSAL, PSB27-1, psbB, three homologs LHCB1.3, Lut 5, mapped gene regulating content also had mutations ym. These 7 shown be significantly differently at one or more time points during accumulation process. It is predicted that 5 candidate accumulation, which results color mutation. identified this can provide new insight into modulation.